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Electrical Stimulation Science Fair Projects

Shocking Medical Treatments for Infections - Electrical Stimulation Science Fair Projects
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Unique treatments for stubborn bacterial infections explored
in high school science fair project...

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Shock Therapies

Electricity can be used for many medical treatments. It can be used to shock a heart back to life or to kill various types of bacterial infections.

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Test bacteria on any surface with the Surface Microbes Science Fair Projects Kit: after hand sanitizer, hands, disinfectant.. for example. You can use antibiotics to test the bacteria too.

Anytime you want to test how many bacteria are On Top of a surface like a desk, skin, chicken, computer keyboard, bread dough, a hard piece of chocolate, cheese, inside of an animal's cheek, etc. then use the surface experimenter kit. The kit will let you calculate how many bacteria there are per unit surface area on the object. You can also test for e-coli, however, only the microbe water kit will let you distinguish e. coli from other coliforms and bacteria.

 

Objectives/Goals

The antibacterial effects of electrical stimulation on bacteria cultures have been examined to see if infections can be prevented or treated. Low-voltage pulsed current has been shown to promote tissue repair in vitro and in vivo. This experiment is focused on inhibitory effects of low voltage elecetricity on Escherichia coli and Staphylococcus epidermidis.

Methods/Materials

Different low voltage amounts were applied to agar plate cultures with stainless steel electrode pairs. The voltage amounts were 0V (0mA), 1.5V (6mA) and 3V (20mA). There were two groups: "Immediate treatment" meaning the agar plate culture was incubated for 24hrs with treatment and the second group was "Delayed treatment" meaning the agar plate culture was incuated for 24hrs (without treatment) and then after 24hrs of growth, it was treated for 24hrs. The results were measured by the area of the zone of inhibition.

Results

Immediate treatment was more effective than delayed treament. In each trial for immediate treatment there was a zone of inhibition at both the anode and the cathode. In the trials of the delayed treatment, there was about a 17-33% chance that there would be a zone of inhibiton around the anode/cathode.

Conclusions/Discussion

There is not a significant difference between th ares of the inhibition zone for E. coli positive/negative electrode and the S. epidermidis positive/negative electrode. 3V and 1.5V showed a difference in the area of the zone of inhibition compared to the 0V (control) in immediate treatment but the voltage amount did not make a difference in delayed treatment. The areas of the inhibition zone around the positive and negative electrodes in immediate treatment yeilded similar areas within its group and so did the delayed treatment. There is a difference between the areas of the ihibition zones for immediate vs. delayed treatment. Immediate treatment was more effective in inhibiting the growth of E. coli and S. epidermidis. 3rd party contributor


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